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Effect of adenosine concentration on quality of cooled ram semen R. Bras. Zootec.
Bezerra,Andréia Santana; Nascimento,Thialla Emille Costa do; Castilho,Erick Fonseca de; Gonçalves,Natália Larissa Cunha; Silva,Sillas Matteus Batista Soares da; Cardoso,Aline Serrão; Souza,Heloíse Karine Rolim de; Rodrigues,Luiz Fernando de Souza.
ABSTRACT This study analyzed the adenosine concentration on quality of ram semen in different refrigerated storage times at 5 °C. The design was in blocks with repeated-measures factorial design. Five levels of adenosine and four refrigeration times were considered as fixed effects and the animal as random effect (block). Ram semen was diluted with Andromed® diluent (control) and increasing adenosine levels (0.5, 0.75, 1, and 1.5%). The samples were taken from refrigeration after 4, 8, 12, and 16 h and evaluated for progressive rectilinear sperm motility, sperm vigor, and membrane integrity (supravital test). Data were subjected to analysis of variance with Tukey's test. The regression analysis was conducted to evaluate behavior of adenosine levels. Factor...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Nucleoside; Oxidative stress; Purinergic; Reproduction; Spermatozoon.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100606
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